Sequencing using pulsed field and image reconstruction. Academic Article

abstract

• The use of pulsed fields in a standard manual sequencing set-up results in the separation of > 2 kb on a single gel, as compared to 300-400 bases with a dc field. However, visual reading of the sequence from a film exposed to a pulsed-field gel is not possible for more than 800-900 bases under the best conditions. The use of image reconstruction and enhancement techniques allows the reading of the M13mp18 sequence to > 1 kb, and individual bands can be identified at > 2 kb.

authors

• Noolandi, J
• Forsyth, R
• Shi, An-chang

status

• published 

publication date

• November 1993

has subject area

• Bacteriophage M13 (MeSH)
• Base Sequence (MeSH)
• DNA (MeSH)
• DNA, Viral (MeSH)
• Electrophoresis, Polyacrylamide Gel (MeSH)
• Genetic Techniques (MeSH)
• Indicators and Reagents (MeSH)
• Mathematics (MeSH)
• Molecular Sequence Data (MeSH)
• Probability (MeSH)
• Templates, Genetic (MeSH)

published in

• Proceedings of the National Academy of Sciences of USA  Journal

keywords

• Bacteriophage M13
• Base Sequence
• DNA
• DNA, Viral
• Electrophoresis, Polyacrylamide Gel
• Genetic Techniques
• Indicators and Reagents
• Mathematics
• Molecular Sequence Data
• Probability
• Templates, Genetic

Digital Object Identifier (DOI)

• 10.1073/pnas.90.21.10101

start page

• 10101

end page

• 10104

volume

• 90

issue

• 21