Journal article
In vivo screening unveils pervasive RNA-binding protein dependencies in leukemic stem cells and identifies ELAVL1 as a therapeutic target
Abstract
Acute myeloid leukemia (AML) is fueled by leukemic stem cells (LSC) whose determinants are challenging to discern from hematopoietic stem cells (HSC) or uncover by approaches focused on general cell properties. We have identified a set of RNA-binding proteins (RBP) selectively enriched in human AML LSCs. Using an in vivo two-step CRISPR-Cas9 screen to assay stem cell functionality, we found 32 RBPs essential for LSCs in MLL-AF9;NrasG12D AML. …
Authors
Vujovic A; de Rooij L; Chahi AK; Chen HT; Yee BA; Loganathan SK; Liu L; Chan DCH; Tajik A; Tsao E
Journal
Blood Cancer Discovery, Vol. 4, No. 3, pp. 180–207
Publisher
American Association for Cancer Research (AACR)
Publication Date
May 1, 2023
DOI
10.1158/2643-3230.bcd-22-0086
ISSN
2643-3230