Journal article
A plasmid vehicle suitable for the molecular cloning and characterization of mammalian promoters
Abstract
We have constructed a plasmid, pPSG4, that carries only the coding sequences for polyomavirus (Py) small, middle and truncated large T-antigens. A unique HindIII site allows the introduction of foreign promoters directly in front of viral coding sequences. The simian virus 40 (SV40) early and late, adenovirus-2 (Ad-2) major late and herpes simplex virus-1 (HSV-1) thymidine kinase (TK) promoters all confer on pPSG4 the ability to transform rat …
Authors
Featherstone MS; Naujokas MA; Pomerantz BJ; Hassell JA
Journal
Nucleic Acids Research, Vol. 12, No. 18, pp. 7235–7249
Publisher
Oxford University Press (OUP)
Publication Date
1984
DOI
10.1093/nar/12.18.7235
ISSN
0305-1048