Journal article
Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies
Abstract
Human pluripotent stem cells (hPSCs) provide an invaluable tool for modeling diseases and hold promise for regenerative medicine. For understanding pluripotency and lineage differentiation mechanisms, a critical first step involves systematically cataloging essential genes (EGs) that are indispensable for hPSC fitness, defined as cell reproduction in this study. To map essential genetic determinants of hPSC fitness, we performed genome-scale …
Authors
Mair B; Tomic J; Masud SN; Tonge P; Weiss A; Usaj M; Tong AHY; Kwan JJ; Brown KR; Titus E
Journal
Cell Reports, Vol. 27, No. 2, pp. 599–615.e12
Publisher
Elsevier
Publication Date
April 2019
DOI
10.1016/j.celrep.2019.02.041
ISSN
2639-1856