Journal article
Total protein or high-abundance protein: Which offers the best loading control for Western blotting?
Abstract
Western blotting routinely involves a control for variability in the amount of protein across immunoblot lanes. Normalizing a target signal to one found for an abundantly expressed protein is widely regarded as a reliable loading control; however, this approach is being increasingly questioned. As a result, we compared blotting for two high-abundance proteins (actin and glyceraldehyde 3-phosphate dehydrogenase [GAPDH]) and two total protein …
Authors
Thacker JS; Yeung DH; Staines WR; Mielke JG
Journal
Analytical Biochemistry, Vol. 496, , pp. 76–78
Publisher
Elsevier
Publication Date
March 2016
DOI
10.1016/j.ab.2015.11.022
ISSN
0003-2697