Sensitive and High-Throughput Analyses of Purine Metabolites by Dynamic pH Junction Multiplexed Capillary Electrophoresis: A New Tool for Metabolomic Studies

On-line sample preconcentration by a dynamic pH junction in conjunction with multiplexed capillary electrophoresis (CE) and UV detection represents a sensitive and high-throughput format for future metabolomic research, such as purine analysis. The optimization of purine focusing can be rapidly assessed by systematically altering the sample matrix properties, such as the buffer co-ion, pH and ionic strength using a 96-capillary array format. …