Journal article
High-throughput screening of holoprotein conformational stability by dynamic ligand exchange-affinity capillary electrophoresis
Abstract
Dynamic ligand exchange-affinity capillary electrophoresis (DLE-ACE) is introduced as a convenient platform for assessing the conformational stability and relative affinity of a holoprotein to different ligands without off-line sample pretreatment, since ligand exchange and protein unfolding processes are integrated in-capillary during electromigration.
Authors
Seguí-Lines G; Gavina JMA; D'Amaral JC; Britz-McKibbin P
Journal
Analyst, Vol. 132, No. 8, pp. 741–744
Publisher
Royal Society of Chemistry (RSC)
Publication Date
August 2007
DOI
10.1039/b705469f
ISSN
0003-2654