Journal article
Circumvention of Fluorophore Photobleaching in Fluorescence Fluctuation Experiments: a Beam Scanning Approach
Abstract
Photobleaching is a fluorophore-damaging process that commonly afflicts single-molecule fluorescence studies. It becomes an especially severe problem in fluorescence fluctuation experiments when studying slowly diffusing particles. One way to circumvent this problem is to use beam scanning to decrease the residence time of the fluorophores in the excitation volume. We report a systematic study of the effects of circular beam scanning on the …
Authors
Satsoura D; Leber B; Andrews DW; Fradin C
Journal
ChemPhysChem, Vol. 8, No. 6, pp. 834–848
Publisher
Wiley
Publication Date
April 23, 2007
DOI
10.1002/cphc.200600589
ISSN
1439-4235