Journal article
Fluorescence correlation spectroscopy with a doughnut-shaped excitation profile as a characterization tool in STED microscopy
Abstract
The resolution of stimulated emission depletion (STED) microscopes is ultimately limited by the quality of the doughnut-shaped illumination profile of the STED erase beam. We show here that in the focal plane this illumination profile is well approximated by an analytical expression - a difference of Gaussian functions, which tends towards a first order Laguerre-Gaussian profile in the case of a well aligned beam with a true zero-intensity …
Authors
Tressler C; Stolle M; Fradin C
Journal
Optics Express, Vol. 22, No. 25, pp. 31154–31166
Publisher
Optica Publishing Group
Publication Date
December 15, 2014
DOI
10.1364/oe.22.031154
ISSN
1094-4087