Journal article
NAD+ ‐dependent malic enzyme of Rhizobium meliloti is required for symbiotic nitrogen fixation
Abstract
DEAE-cellulose chromatography of extracts of free-living Rhizobium meliloti cells revealed separate NAD(+)-dependent and NADP(+)-dependent malic enzyme activities. The NAD+ malic enzyme exhibited more activity with NAD+ as cofactor, but also showed some activity with NADP+. The NADP+ malic enzyme only showed activity when NADP+ was supplied as cofactor. Three independent transposon-induced mutants of R. meliloti which lacked NAD+ malic enzyme …
Authors
Driscoll BT; Finan TM
Journal
Molecular Microbiology, Vol. 7, No. 6, pp. 865–873
Publisher
Wiley
Publication Date
March 1993
DOI
10.1111/j.1365-2958.1993.tb01177.x
ISSN
0950-382X