Journal article
Tn5 mutagenesis of the transforming genes of human adenovirus type 5
Abstract
We have cloned the entire human adenovirus type 5 (Ad5) genome into the pBR322 plasmid in two segments: the BamHI-A fragment (21 kb) and the BamHI-B fragment (15 kb). We have also generated a series of clones with smaller Ad5 DNA inserts, all containing the left-end of the viral genome. One such clone, pXCl, containing the left 16% of the Ad5 DNA molecule, has been shown to transform rodent cells by DNA transfection. We have used the …
Authors
McKinnon RD; Bacchetti S; Graham FL
Journal
Gene, Vol. 19, No. 1, pp. 33–42
Publisher
Elsevier
Publication Date
7 1982
DOI
10.1016/0378-1119(82)90186-x
ISSN
0378-1119