Growth of 293 Cells in Suspension Culture

Abstract

A subline of 293 cells able to grow in suspension culture has been developed by passage of 293 cells through nude mice. This new line, designated 293N3S, grows with a doubling time of approximately 30 h, continues to express adenovirus 5 early region 1 (E1) antigens, and remains permissive for adenovirus 5 host range mutants defective in E1 functions.

Authors

Graham FL

Journal

Journal of General Virology, Vol. 68, No. 3, pp. 937–940

Publisher

Microbiology Society

Publication Date

March 1, 1987

DOI

10.1099/0022-1317-68-3-937

ISSN

0022-1317

Associated Experts

Frank Lawson Graham

Professor Emeritus, Faculty of Science

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