Growth of 293 Cells in Suspension Culture Journal Articles

abstract

• A subline of 293 cells able to grow in suspension culture has been developed by passage of 293 cells through nude mice. This new line, designated 293N3S, grows with a doubling time of approximately 30 h, continues to express adenovirus 5 early region 1 (E1) antigens, and remains permissive for adenovirus 5 host range mutants defective in E1 functions.

authors

• Graham, Frank Lawson

status

• published 

publication date

• March 1, 1987

has subject area

• 06 Biological Sciences (FoR)
• 07 Agricultural and Veterinary Sciences (FoR)
• 11 Medical and Health Sciences (FoR)
• Adenoviruses, Human (MeSH)
• Animals (MeSH)
• Antigens, Neoplasm (MeSH)
• Cell Line (MeSH)
• Cell Transformation, Neoplastic (MeSH)
• Culture Techniques (MeSH)
• Embryo, Mammalian (MeSH)
• Humans (MeSH)
• Mice (MeSH)
• Mice, Nude (MeSH)
• Neoplasm Transplantation (MeSH)
• Transplantation, Heterologous (MeSH)
• Virology (Science Metrix)

published in

• Journal of General Virology  Journal

keywords

• Adenoviruses, Human
• Animals
• Antigens, Neoplasm
• Cell Line
• Cell Transformation, Neoplastic
• Culture Techniques
• Embryo, Mammalian
• Humans
• Mice
• Mice, Nude
• Neoplasm Transplantation
• Transplantation, Heterologous

Digital Object Identifier (DOI)

• 10.1099/0022-1317-68-3-937

PubMed ID

• 3819703

start page

• 937

end page

• 940

volume

• 68

issue

• 3