Adenovirus E1A under the control of heterologous promoters: Wide variation in E1A expression levels has little effect on virus replication

Adenovirus early region 1A(E1A) encodes a heterogeneous family of proteins some of which function as transactivators and are required for efficient viral replication in HeLa cells. We have constructed adenovirus type 5 (Ad 5) mutants in which the E1A transcription unit is placed under the control of either the human beta-actin promoter or the human cytomegalovirus immediate early promoter. The level of E1A expression in cells infected with …