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A helper-dependent adenovirus vector system:...
Journal article

A helper-dependent adenovirus vector system: Removal of helper virus by Cre-mediated excision of the viral packaging signal

Abstract

Adenoviruses are attractive vectors for the delivery of foreign genes into mammalian cells for gene therapy. However, current vectors retain many viral genes that, when expressed at low levels, contribute to the induction of a host immune response against transduced cells. We have developed a helper-dependent packaging system for production of vectors that have large regions of the genome deleted. Helper viruses were constructed with packaging …

Authors

Parks RJ; Chen L; Anton M; Sankar U; Rudnicki MA; Graham FL

Journal

Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 24, pp. 13565–13570

Publisher

Proceedings of the National Academy of Sciences

Publication Date

November 26, 1996

DOI

10.1073/pnas.93.24.13565

ISSN

0027-8424