Journal article
Cre Levels Limit Packaging Signal Excision Efficiency in the Cre/loxP Helper-Dependent Adenoviral Vector System
Abstract
Helper-dependent (HD) adenovirus vectors devoid of all viral coding sequences have a large cloning capacity and provide long-term transgene expression in vivo with negligible toxicity, making them attractive vectors for gene therapy. Currently, the most efficient means of producing HD vectors involves coinfecting 293 cells expressing Cre with the HD vector and a helper virus bearing a packaging signal flanked by loxP sites. Cre-mediated …
Authors
Ng P; Evelegh C; Cummings D; Graham FL
Journal
Journal of Virology, Vol. 76, No. 9, pp. 4181–4189
Publisher
American Society for Microbiology
Publication Date
May 2002
DOI
10.1128/jvi.76.9.4181-4189.2002
ISSN
0022-538X