Journal article
Functional rescue of vitamin C synthesis deficiency in human cells using adenoviral-based expression of murine l-gulono-γ-lactone oxidase
Abstract
l-Gulono-gamma-lactone oxidase (GULO) is a critical enzyme present in most mammalian species that is required for the terminal step in vitamin C biosynthesis. Primates are absolutely dependent on exogenously supplied dietary vitamin C due to inactivation of the Gulo gene by mutation over 40 million years ago. In this study, we report the cloning and expression of the murine l-gulono-gamma-lactone oxidase cDNA and gene. The cDNA (2.3 kb) encodes …
Authors
Ha MN; Graham FL; D'Souza CK; Muller WJ; Igdoura SA; Schellhorn HE
Journal
Genomics, Vol. 83, No. 3, pp. 482–492
Publisher
Elsevier
Publication Date
3 2004
DOI
10.1016/j.ygeno.2003.08.018
ISSN
0888-7543