A rapid method for the radioimmunoassay of urinary aldosterone

1.The availability of an antiserum to aldosterone γ-lactone allows the rapid assay of aldosterone in urine (1).2.Following acid hydrolysis of the 18 oxo-conjugate, aldosterone is oxidized to its γ-lactone.3.The extremely low cross reactivity of the antiserum with structurally related steroids obviates the need for chromatographic purification before radio-immunoassay.4.This procedure is sensitive enough to allow the assay of aldosterone in the equivalent of 25 UL of hydrolyzed urine.