Journal article
Measuring the Lateral Diffusion of Plasma Membrane Receptors Using Raster Image Correlation Spectroscopy
Abstract
Raster image correlation spectroscopy (RICS) enables detecting and quantifying diffusion in live cells using standard commercial laser scanning confocal microscopes. Here, we describe a protocol based on RICS for measuring the lateral diffusion of two immunoreceptors within the plasma membrane of the macrophage cell line RAW 264.7. The sample images and measurements presented in this chapter were obtained from RICS analysis of Toll-like …
Authors
Makaremi S; Moran-Mirabal J
Journal
Methods in Molecular Biology, Vol. 2440, , pp. 289–303
Publisher
Springer Nature
Publication Date
2022
DOI
10.1007/978-1-0716-2051-9_17
ISSN
1064-3745