Survival of human islets in microbeads containing high guluronic acid alginate crosslinked with Ca2+and Ba2+ Academic Article uri icon

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  • Background

    The main hurdles to the widespread use of islet transplantation for the treatment of type 1 diabetes continue to be the insufficient number of appropriate donors and the need for immunosuppression. Microencapsulation has been proposed as a means to protect transplanted islets from the host's immune system.


    This study investigated the function of human pancreatic islets encapsulated in Ca(2+) /Ba(2+) -alginate microbeads intraperitoneally transplanted in diabetic Balb/c mice.


    All mice transplanted with encapsulated human islets (n = 29), at a quantity of 3000 islet equivalent (IEQ), achieved normoglycemia 1 day after transplantation and retained normoglycemia for extended periods of time (mean graft survival 134 ± 17 days). In comparison, diabetic Balb/c mice transplanted with an equal amount of non-encapsulated human islets rejected the islets within 2 to 7 days after transplantation (n = 5). Microbeads retrieved after 232 days (n = 3) were found with little to no fibrotic overgrowth and contained viable insulin-positive islets. Immunofluorescent staining on the retrieved microbeads showed F4/80-positive macrophages and alpha smooth muscle actin-positive fibroblasts but no CD3-positive T lymphocytes.


    The Ca(2+) /Ba(2+) -alginate microbeads can protect human islets from xenogeneic rejection in immunocompetent mice without immunosuppression. However, grafts ultimately failed likely secondary to a macrophage-mediated foreign body reaction.


  • Qi, Meirigeng
  • Mørch, Yrr
  • Lacík, Igor
  • Formo, Kjetil
  • Marchese, Enza
  • Wang, Yong
  • Danielson, Kirstie K
  • Kinzer, Katie
  • Wang, Shusen
  • Barbaro, Barbara
  • Kolláriková, Gabriela
  • Chorvát, Dušan
  • Hunkeler, David
  • Skjåk-Braek, Gudmund
  • Oberholzer, José
  • Strand, Berit L

publication date

  • November 2012