Oxidative stress in cells exposed to low levels of ionizing radiation Journal Articles

abstract

• The ability of medium from γ-irradiated cells to induce early events in the apoptotic cascade, such as the mobilization of intracellular calcium, loss of mitochondrial membrane potential and increased levels of reactive oxygen species, in unirradiated cells was investigated. Medium from irradiated human keratinocytes was harvested and transferred to unirradiated keratinocytes. Intracellular calcium levels, mitochondrial membrane potential and the level of reactive oxygen species were all monitored for a period of 24 h following medium transfer. Rapid calcium fluxes (within 30 s), loss of mitochondrial membrane potential and increases in reactive oxygen species (from 6 h after medium transfer) were observed. There was no significant difference between the effects of medium generated by cells irradiated at 0.5 Gy or 5 Gy. The data suggest that a signal that leads to apoptosis is released from cells undergoing radiation-induced oxidative stress.

authors

• Lyng, FM
• Seymour, Colin
• Mothersill, Carmel Emelia

status

• published 

publication date

• May 1, 2001

has subject area

• 0601 Biochemistry and Cell Biology (FoR)
• 1101 Medical Biochemistry and Metabolomics (FoR)
• Apoptosis (MeSH)
• Biochemistry & Molecular Biology (Science Metrix)
• Calcium (MeSH)
• Cell Line (MeSH)
• Gamma Rays (MeSH)
• Humans (MeSH)
• Keratinocytes (MeSH)
• Membrane Potentials (MeSH)
• Mitochondria (MeSH)
• Oxidative Stress (MeSH)
• Reactive Oxygen Species (MeSH)

published in

• Biochemical Society Transactions  Journal

keywords

• Apoptosis
• Calcium
• Cell Line
• Gamma Rays
• Humans
• Keratinocytes
• Membrane Potentials
• Mitochondria
• Oxidative Stress
• Reactive Oxygen Species

Digital Object Identifier (DOI)

• 10.1042/bst0290350

PubMed ID

• 11356181

start page

• 350

end page

• 353

volume

• 29

issue

• 2