Journal article
Amplicon pyrosequencing late Pleistocene permafrost: the removal of putative contaminant sequences and small‐scale reproducibility
Abstract
DNA sequencing of ancient permafrost samples can be used to reconstruct past plant, animal and bacterial communities. In this study, we assess the small-scale reproducibility of taxonomic composition obtained from sequencing four molecular markers (mitochondrial 12S ribosomal DNA (rDNA), prokaryote 16S rDNA, mitochondrial cox1 and chloroplast trnL intron) from two soil cores sampled 10 cm apart. In addition, sequenced control reactions were …
Authors
Porter TM; Golding GB; King C; Froese D; Zazula G; Poinar HN
Journal
Molecular Ecology Resources, Vol. 13, No. 5, pp. 798–810
Publisher
Wiley
Publication Date
September 2013
DOI
10.1111/1755-0998.12124
ISSN
1755-098X