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Known Bioactive Small Molecules Probe the Function...
Journal article

Known Bioactive Small Molecules Probe the Function of a Widely Conserved but Enigmatic Bacterial ATPase, YjeE

Abstract

Escherichia coli YjeE is a broadly conserved bacterial ATPase of unknown function that has been widely characterized as essential. Here, the transcriptional regulation of the promoter of yjeE (P(yjeE)) was probed using a luciferase reporter and 172 antibiotics of diverse mechanisms. Norfloxacin and other fluorquinolones were found to be the most potent activator of P(yjeE) through binding to DNA gyrase. The stimulation of P(yjeE) by norfloxacin …

Authors

Mangat CS; Brown ED

Journal

Cell Chemical Biology, Vol. 15, No. 12, pp. 1287–1295

Publisher

Elsevier

Publication Date

December 2008

DOI

10.1016/j.chembiol.2008.10.007

ISSN

2451-9456