Disturbance in Na+ regulation in cells rich in mitochondria isolated from gills of the yellow clam Mesodesma mactroides exposed to copper under different osmotic conditions

Cells rich in mitochondria were isolated from gills of the seawater clam Mesodesma mactroides, incubated in isosmotic saline solution (840 mOsmol/kg H₂O), and exposed (3 h) to environmentally realistic Cu concentrations (nominally: 0, 5, 9 and 20 μg/L). In cells exposed to 20 μg Cu/L, Cu accumulation, Na⁺ content reduction and carbonic anhydrase (CA) activity inhibition were observed, without significant changes in cell viability and Na⁺,K⁺-ATPa…