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In Vitro Selection of Circular DNA Aptamers for...
Journal article

In Vitro Selection of Circular DNA Aptamers for Biosensing Applications

Abstract

Abstract We report on the first effort to select DNA aptamers from a circular DNA library, which resulted in the discovery of two high‐affinity circular DNA aptamers that recognize the glutamate dehydrogenase (GDH) from Clostridium difficile, an established antigen for diagnosing Clostridium difficile infection (CDI). One aptamer binds effectively in both the circular and linear forms, the other is functional only in the circular configuration. …

Authors

Liu M; Yin Q; Chang Y; Zhang Q; Brennan JD; Li Y

Journal

Angewandte Chemie, Vol. 131, No. 24, pp. 8097–8101

Publisher

Wiley

Publication Date

June 11, 2019

DOI

10.1002/ange.201901192

ISSN

0044-8249