Preparation of Helper-Dependent Adenoviral Vectors

Adenoviruses (Ads) are excellent mammalian gene transfer vectors because of their ability to infect efficiently a wide variety of quiescent and proliferating cell types from various species to direct high-level gene expression. Consequently, Ad vectors are extensively used as potential recombinant viral vaccines, for high-level protein production in cultured cells and for gene therapy (1–4). First-generation Ad vectors typically have foreign …