From model system to therapy ⇓ scalable production of perfusable vascularized liver spheroids in ⇜open-top⇝ 384-well plate

Here we demonstrate the scalable production of perfusable and extractable vascularized tissues in an “open-top“384-well plate (referred to as IFlowPlateTM), showing that this system could be used to examine nanoparticle delivery to targeted tissues through the microvascular network and to model vascular angiogenesis. Furthermore, tissue spheroids, such as hepatic spheroids, can be vascularized in a scalable manner and then subsequently extracted for in vivo implantation. This simple multiple-well plate platform could not only improve the experimental throughputs of organ-on-a-chip systems but could potentially help expand the application of model systems to regenerative therapy.