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Single-Step Printable Hydrogel Microarray...
Journal article

Single-Step Printable Hydrogel Microarray Integrating Long-Chain DNA for the Discriminative and Size-Specific Sensing of Nucleic Acids

Abstract

A simple approach to fabricating hydrogel-based DNA microarrays is reported by physically entrapping the rolling circle amplification (RCA) product inside printable in situ gelling hydrazone cross-linked poly(oligoethylene glycol methacrylate) hydrogels. The hydrogel-printed RCA microarray facilitates improved RCA immobilization (>65% even after vigorous washing) and resistance to denaturation relative to RCA-only printed microarrays in …

Authors

Pikula M; Ali MM; Filipe C; Hoare T

Journal

ACS Applied Materials & Interfaces, Vol. 13, No. 2, pp. 2360–2370

Publisher

American Chemical Society (ACS)

Publication Date

January 20, 2021

DOI

10.1021/acsami.0c21061

ISSN

1944-8244