An NMR based phosphodiesterase assay Journal Articles

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abstract

A phosphodiesterase (PDE) assay based on 1D¹H NMR to monitor the hydrolysis of cyclic nucleotides directly, without requiring tags or the addition of exogenous reagents.

authors

Akimoto, Madoka
Yu, Tianning
Moleschi, Kody
Van, Katherine
Anand, Ganesh S
Melacini, Giuseppe

publication date

July 25, 2020

has subject area

03 Chemical Sciences (FoR)
Enzyme Assays (MeSH)
Enzyme Inhibitors (MeSH)
Hydrolysis (MeSH)
Molecular Structure (MeSH)
Nuclear Magnetic Resonance, Biomolecular (MeSH)
Nucleotides (MeSH)
Organic Chemistry (Science Metrix)
Phosphoric Diester Hydrolases (MeSH)

published in

Chemical Communications Journal