Journal article
Quantitation of Chlamydia trachomatis 16S rRNA using NASBA amplification and a bioluminescent microtiter plate assay.
Abstract
We developed a nucleic acid sequence based amplification (NASBA) assay which employs the recombinant photoprotein Aequorin in a microtiter plate format for detection and quantitation of C. trachomatis that may be useful in large scale epidemiological studies aimed at improving our understanding of factors affecting transmission of this sexually transmitted pathogen. The conditions for NASBA amplification of the16S rRNA target were optimized (90 …
Authors
Song X; Coombes BK; Mahony JB
Journal
Combinatorial Chemistry & High Throughput Screening, Vol. 3, No. 4, pp. 303–313
Publisher
Bentham Science Publishers
Publication Date
August 2000
DOI
10.2174/1386207003331571
ISSN
1386-2073