Flow cytometric measurement of intracellular IFN-gamma induction in aged subjects before and after parenteral influenza vaccination. Academic Article uri icon

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abstract

  • Flow cytometric analysis, used to study intracellular expression of IFN-gamma in peripheral blood mononuclear cells (PBMC) from aged volunteers before and after parenteral influenza vaccination, was found capable of rapidly detecting influenza antigen induced variation of IFN-gamma expression. Although the vaccine was capable of generating a satisfactory antibody response, it did not stimulate an increase in the percentage of IFN-gamma positive cells.

authors

  • Fringuelli, E
  • Lepri, E
  • Camilloni, B
  • Neri, M
  • CarriĆ², E
  • Russano, A
  • Spinozzi, F
  • Iorio, Alfonso

publication date

  • 2003