The Sinorhizobium meliloti chromosomal origin of replication Journal Articles

abstract

• The predicted chromosomal origin of replication (oriC) from the alfalfa symbiontSinorhizobium melilotiis shown to allow autonomous replication of a normally non-replicating plasmid withinS. meliloticells. This is the first chromosomal replication origin to be experimentally localized in theRhizobiaceaeand its location, adjacent tohemE, is the same as fororiCinCaulobacter crescentus, the only experimentally characterized alphaproteobacterialoriC. Using an electrophoretic mobility shift assay and purifiedS. melilotiDnaA replication initiation protein, binding sites for DnaA were mapped in theS. meliloti oriCregion. Mutations in these sites eliminated autonomous replication.S. melilotithat expressed DnaA from a plasmidlacpromoter was observed to form pleomorphic filamentous cells, suggesting that cell division was perturbed. Interestingly, this cell phenotype is reminiscent of differentiated bacteroids found inside plant cells in alfalfa root nodules.

authors

• Sibley, Christopher D
• MacLellan, Shawn R
• Finan, Turlough Mary

status

• published 

publication date

• February 1, 2006

has subject area

• Bacterial Proteins (MeSH)
• Binding Sites (MeSH)
• Cell Cycle (MeSH)
• Chromosomes, Bacterial (MeSH)
• DNA Replication (MeSH)
• DNA, Bacterial (MeSH)
• DNA-Binding Proteins (MeSH)
• Microbiology (Science Metrix)
• Replication Origin (MeSH)
• Sinorhizobium meliloti (MeSH)

published in

• Microbiology (United Kingdom)  Journal

keywords

• Bacterial Proteins
• Binding Sites
• Cell Cycle
• Chromosomes, Bacterial
• DNA Replication
• DNA, Bacterial
• DNA-Binding Proteins
• Replication Origin
• Sinorhizobium meliloti

Digital Object Identifier (DOI)

• 10.1099/mic.0.28455-0

PubMed ID

• 16436432

start page

• 443

end page

• 455

volume

• 152

issue

• 2