Micropatterned biofunctional lubricant-infused surfaces promote selective cell adhesion and patterning

We report development of micropatterned biofunctional lubricant-infused surfaces which allows simultaneous repellency, biofunctionality, and targeted binding. We demonstrate specific adhesion of red fluorescent protein expressing human umbilical vein endothelial cells (RFP-HUVECs) to a lubricant-infused surface micropatterned with an endothelial cell specific antibody, anti-CD34. Our proposed blocking method was compared to conventional ones, such as bovine serum albumin (BSA) or poly(ethylene glycol)-based materials (e.g. PLLPEG) and also, its capture capability was evaluated in a complex biological system (e.g. blood). The developed surfaces yield a superior targeted adhesion of cells which can further be utilized in applications such as biosensors or implants.