A microfluidic microinjection of drosophila embryo in a format using compliant mechanism and electrokinetic dosage control

Microinjection is an established and reliable method to deliver transgenic constructs and other reagents to specific locations in the Drosophila's embryo. Although, current embryo microinjection method is effective in creating transgenic flies, it requires expensive multi degree of freedom micromanipulator, detailed alignment procedure of the posterior and skilled operator which makes the microinjection process slow and not suitable for high throughput. In this paper, the first PDMS-based microinjection system in a microfluidic format with accurate electrokinetic flow control for the Drosophila's embryo injection, has been presented. The device design is completely scalable and enables high-throughput microinjections with integrated pre- and post-processing operations.