Characterization of epidermal growth factor-modified polydimethyl siloxane
Abstract
The binding of pegylated epidermal growth factor (EGF) to polydimethyl siloxane (PDMS) substrates for enhancement of corneal epithelial cell proliferation was discussed. The SPR and radiolabeling were used to measure the binding of pegylated EGF to modified gold-PDMS-allylamine surfaces. It was found that the SPR signal was correlated with binding levels obtained by radiolabelling. Analysis suggested that optimization of reaction conditions is expected to increase the amount of covalently bound EGF and subsequent enhancement of epithelial cell growth.