Peptide modified gold coated polyurethanes for thrombin inhibition

The effects of peptide modified gold coated polyurethanes on thrombin adsorption and thrombin inhibition were examined. The polyurethanes were chemisorbed with a thiol containing thrombin inhibiting peptide. Thrombin adsorption to the polyurethane films was measured from plasma at different dilutions and from phosphate buffered saline. The biotinylated thrombin on the surface was detected by binding to alkaline phosphatase conjugated streptavidin followed by color generation using p-nitrophenyl phosphate. Thrombin activity following incubation with the surfaces was measured using a chromogenic substrate assay. Results indicate that longer chemisorption times may result in greater ligand surface density and greater levels of adsorption and inhibition.