INTERACTIONS OF PLASMA WITH GLASS: IDENTIFICATION OF ADSORBED PROTEINS.

Although it is widely recognized that blood-material interactions leading to thrombosis are initiated by protein adsorption there is little or no information regarding the identity of the proteins in the adsorbed layer. Most studies have emphasized the more abundant proteins and adsorption data have been obtained by trace-labelling techniques. However it has been shown that the most abundant proteins, e. g. albumin, fibrinogen and IgG, are adsorbed only to a limited extent, suggesting that less abundant proteins may be important components of adsorbed layers. The present paper describes an attempt to identify proteins adsorbed from plasma to glass using a chromatographic column approach. Plasma is first incubated on the column and then washed out. Adsorbed proteins are then eluted and identified by gel electrophoresis and immunoassay.