The structural basis for the serospecificity ofActinobacillus suisserogroup O:2 Academic Article uri icon

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abstract

  • Actinobacillus suis is an important bacterial pathogen of healthly pigs. An O-antigen (lipopolysaccharide; LPS) serotyping system is being developed to study the prevalence and distribution of representative isolates from both healthy and diseased pigs. In a previous study, we reported that A. suis serogroup O:1 strains express LPS with a (1→6)-β-D-glucan O-antigen chain polysaccharide that is similar in structure to a key cell-wall component in yeasts, such as Saccharomyces cerevisiae and Candida albicans. This study describes the O-antigen polysaccharide chemical structure of an O:2 serogroup strain, A. suis H91-0380, which possesses a tetrasaccharide repeating block with the structure: →3)-β-D-Galp-(1→4)-[α-D-Galp-(1→6)]-β-D-Glcp-(1→6)-β-D-GlcpNAc-(1→. Studies have shown that A. suis serogroup O:2 strains are associated with severely diseased animals; therefore, work on the synthesis of a glycoconjugate vaccine employing O:2 O-antigen polysaccharide to vaccinate pigs against A. suis serogroup O:2 strains is currently underway.Key words: Actinobacillus suis, lipopolysaccharide, serogroup O:2, vaccine.

authors

  • Rullo, Anthony
  • Papp-Szabo, Erzsebet
  • Michael, Frank St
  • MacInnes, Janet
  • Monteiro, Mario A

publication date

  • April 1, 2006

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