Evaluation of horseradish peroxidase as an intracellular stain in electrophysiological recording
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Iontophoresis of horseradish peroxidase into cells during intracellular electrophysiological recording to visualize the neurons recorded from is a powerful technique, but its success depends on a number of critical factors. The described agar injection technique is a simple method for the evaluation of several parameters at once, using procedures which closely resemble recording from, fixing, and processing to visualize the HRP in experimental tissue.
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