Applications of CRISPR-Cas in its natural habitat Journal Articles

abstract

• Key components of CRISPR-Cas systems have been adapted into a powerful genome-editing tool that has caught the headlines and the attention of the public. Canonically, a customized RNA serves to guide an endonuclease (e.g. Cas9) to its DNA target, resulting in precise genomic lesions that can be repaired in a personalized fashion by cellular machinery. Here, we turn to the microbes that are the source of this system to explore many of its other notable applications. These include mining the CRISPR 'memory' arrays for functional genomic data, generation of customized virus-resistant or plasmid-refractory bacterial cells, editing of previously intractable viral genomes, and exploiting the unique properties of a catalytically inactive Cas9, dCas9, to serve as a highly customizable anti-nucleic acid 'antibody'.

authors

• Hynes, Alexander
• Lemay, Marie-Laurence
• Moineau, Sylvain

status

• published 

publication date

• October 2016

has subject area

• 0304 Medicinal and Biomolecular Chemistry (FoR)
• 0601 Biochemistry and Cell Biology (FoR)
• Bacteria (MeSH)
• Bacteriophages (MeSH)
• CRISPR-Cas Systems (MeSH)
• Genome, Bacterial (MeSH)
• Genome, Viral (MeSH)
• Organic Chemistry (Science Metrix)

published in

• Current Opinion in Chemical Biology  Journal

keywords

• Bacteria
• Bacteriophages
• CRISPR-Cas Systems
• Genome, Bacterial
• Genome, Viral

Digital Object Identifier (DOI)

• 10.1016/j.cbpa.2016.05.021

PubMed ID

• 27280696

start page

• 30

end page

• 36

volume

• 34