Journal article
Assessment of methylation level prediction accuracy in methyl-DNA immunoprecipitation and sodium bisulfite based microarray platforms
Abstract
In this study, we verified the accuracy of two array methods--methylated DNA immunoprecipitation coupled with CpG island microarrays (MeDIP-CGI-arrays) and sodium bisulfite conversion based microarrays (BC-arrays)--in predicting regional methylation levels as measured by pyrosequencing of bisulfite converted DNA (BC-pyrosequencing). To test the accuracy of these methods we used the Agilent Human CpG island and the Illumina HumanMethylation27 …
Authors
Rajendram R; Ferreira JC; Grafodatskaya D; Choufani S; Chiang T; Pu S; Butcher DT; Wodak SJ; Weksberg R
Journal
Epigenetics, Vol. 6, No. 4, pp. 410–415
Publisher
Taylor & Francis
Publication Date
April 2011
DOI
10.4161/epi.6.4.14763
ISSN
1559-2294