Dinitropyrene-resistant Salmonella typhimurium are deficient in an acetyl-CoA acetyltransferase
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abstract
Salmonella typhimurium strain TA98NR which is sensitive to 1,8-dinitropyrene mutagenesis possesses acetyl-CoA dependent acetyltransferase activity, while a strain selected for resistance to 1,8-dinitropyrene (TA98/1, 8-DNP6) is deficient in this activity. Acetyltransferase competent strains can acetylate 1,8-diaminopyrene, forming 1-N-acetylamino-8-aminopyrene and 1,8-N,N'-diacetyldiaminopyrene. The coincidence of dinitropyrene resistance and acetyltransferase deficiency implicates acetylation as an important process in the metabolic activation of dinitropyrene to a mutagenic intermediate.