The 1-nitropyrene reductase of Salmonella...

The 1-nitropyrene reductase of Salmonella typhimurium

Abstract

We have devised a sensitive fluorimetric assay to monitor the conversion of 1-nitropyrene to 1-aminopyrene. Application of this assay to extracts of Salmonella typhimurium strains TA98 and TA100 (which are sensitive to the mutagenic and lethal effects of 1-nitropyrene) has shown that these bacteria contain 'nitropyrene reductase' activity at the low level of 10(-11) mol/min/mg protein. NADPH and NADH serve equally well as reducing agents. The nitropyrene reductase activity of strain TA100 F50 (a mutant resistant to 1-nitropyrene) was found to be considerably lower.

Authors

Lu C; McCalla DR; McCarry BE

Journal

Chemico-Biological Interactions, Vol. 43, No. 1, pp. 67–71

Publisher

Elsevier

Publication Date

January 1, 1983

DOI

10.1016/0009-2797(83)90104-7

ISSN

0009-2797

Associated Experts

Dennis Robert Mccalla

Professor Emeritus, Faculty of Health Sciences

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