Journal article
Taming of a Poison: Biosynthesis of the NiFe-Hydrogenase Cyanide Ligands
Abstract
NiFe-hydrogenases have an Ni-Fe site in which the iron has one CO and two CN groups as ligands. Synthesis of the CN ligands requires the activity of two hydrogenase maturation proteins: HypF and HypE. HypF is a carbamoyltransferase that transfers the carbamoyl moiety of carbamoyladenylate to the COOH-terminal cysteine of HypE and thus forms an enzyme-thiocarbamate. HypE dehydrates the S-carbamoyl moiety in an adenosine triphosphate-dependent …
Authors
Reissmann S; Hochleitner E; Wang H; Paschos A; Lottspeich F; Glass RS; Böck A
Journal
Science, Vol. 299, No. 5609, pp. 1067–1070
Publisher
American Association for the Advancement of Science (AAAS)
Publication Date
February 14, 2003
DOI
10.1126/science.1080972
ISSN
0036-8075