Journal article
Properties of the earliest clonogenic hemopoietic precursors to appear in the developing murine yolk sac.
Abstract
We have found that a variety of clonogenic hemopoietic cells can be obtained in a viable state from mouse conceptuses as early as day 7 of gestation when tissues are disaggregated in a crude collagenase solution containing fetal bovine serum. Examination of the time course of colony formation, and the ultimate size and lineages represented in colonies produced in semisolid medium containing methylcellulose, together with analysis of individual …
Authors
Wong PM; Chung SW; Chui DH; Eaves CJ
Journal
Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 11, pp. 3851–3854
Publisher
Proceedings of the National Academy of Sciences
Publication Date
6 1986
DOI
10.1073/pnas.83.11.3851
ISSN
0027-8424