A multiplexable assay for screening antibiotic lethality against drug-tolerant bacteria Academic Article uri icon

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abstract

  • Antibiotic screens typically rely on growth inhibition to characterize compound bioactivity-an approach that cannot be used to assess the bactericidal activity of antibiotics against bacteria in drug-tolerant states. To address this limitation, we developed a multiplexed assay that uses metabolism-sensitive staining to report on the killing of antibiotic-tolerant bacteria. This method can be used with diverse bacterial species and applied to genome-scale investigations to identify therapeutic targets against tolerant pathogens.

authors

  • Stokes, Jonathan M
  • Gutierrez, Arnaud
  • Lopatkin, Allison J
  • Andrews, Ian W
  • French, Shawn
  • Matic, Ivan
  • Brown, Eric
  • Collins, James J

publication date

  • April 2019