Characterization of culture-induced cytotoxicity from human peripheral lymphocyte subpopulations

Cultured human lymphocyte subpopulations can generate cytotoxicity against K562 leukemia target cells under certain conditions. Such cytotoxicity arises during mixed leukocyte culture or in medium containing fetal calf serum (FCS), mitogenic factors, or interleukin 2. We cultured peripheral blood lymphocytes (PBL) in FCS-containing medium after fractionation of these cells on a Percoll discontinuous density gradient. Higher density cell fractions generated culture-induced spontaneous cytotoxicity (CIC) after 2-3 days in culture. CIC was not due to a loss of suppressor cells during fractionation since culture of PBL prior to fractionation yielded the same results. At least some CIC was associated with the differentiation of higher density cells to newly appearing lower density cells during culture. Most CIC required cells with the HNK-1- OKT3- OKM1+ phenotype. Culture-induced cytotoxicity has some similarities to the previously described lymphokine-activated killing but some important differences are also discussed.