Journal article
Freeze-substitution staining of rat growth plate cartilage with alcian blue for electron microscopic study of proteoglycans.
Abstract
To selectively stain polyanionic macromolecules of growth plate cartilage and to prevent artifacts induced by aqueous fixation, proximal tibial growth plates were excised from rats, slam-frozen, and freeze-substituted in 100% methanol containing the cationic dye Alcian blue. Electron microscopic examination showed the tissue stained with Alcian blue to be comparable in ultrastructural preservation to tissues slam-frozen and freeze-substituted …
Authors
Maitland ME; Arsenault AL
Journal
Journal of Histochemistry & Cytochemistry, Vol. 37, No. 3, pp. 383–387
Publisher
SAGE Publications
Publication Date
March 1989
DOI
10.1177/37.3.2465336
ISSN
0022-1554