Journal article
Development and validation of probe-based multiplex real-time PCR assays for the rapid and accurate detection of freshwater fish species
Abstract
Reliable species identification methods are important for industrial environmental monitoring programs. Probe based real-time quantitative polymerase chain reaction (qPCR) provides an accurate, cost-effective and high-throughput method for species identification. Here we present the development and validation of species-specific primers and probes for the cytochrome c oxidase (COI) gene for the identification of eight ecologically and …
Authors
Hulley EN; Tharmalingam S; Zarnke A; Boreham DR
Journal
PLOS ONE, Vol. 14, No. 1,
Publisher
Public Library of Science (PLoS)
DOI
10.1371/journal.pone.0210165
ISSN
1932-6203