Acute effects of LH on incorporation of [35S]-methionine into proteins by isolated rabbit ovarian follicles

The incorporation of [35S]-methionine into follicular protein was investigated during 5 min intervals up to 20 min, and correlated with testosterone production. At 5 and 20 min after stimulation with luteinizing hormone (LH) there was no difference in the [35S]-labelled proteins from the controls. At 10 and 15 min, LH seemed to decrease the intensity of a protein band with a molecular weight of approximately 25,000 daltons. There was no …