Acute effects of LH on incorporation of [35S]-methionine into proteins by isolated rabbit ovarian follicles

The incorporation of [35S]-methionine into follicular protein was investigated during 5 min intervals up to 20 min, and correlated with testosterone production. At 5 and 20 min after stimulation with luteinizing hormone (LH) there was no difference in the [35S]-labelled proteins from the controls. At 10 and 15 min, LH seemed to decrease the intensity of a protein band with a molecular weight of approximately 25,000 daltons. There was no significant difference in the amount of TCA precipitable radioactivity between control and LH treated follicles. Testosterone production increased within 10 min of stimulation with LH. Under the conditions employed these data suggest that repression of a an inhibitor protein may be required for testosterone production by isolated rabbit ovarian follicles.