STEROID PRODUCTION BY ISOLATED RABBIT OVARIAN FOLLICLES: EFFECTS OF LUTEINIZING HORMONE FROM MATING TO IMPLANTATION

Follicles were isolated from rabbits before and after mating and the effects of LH on steroidogenesis were studied using an incubation technique. Before mating testosterone was the major steroid produced in response to LH. Mating or administration of ovine LH in vivo caused the follicles to produce mainly progesterone and these follicles were refractory to LH in vitro. Up to 72 h after mating, LH would stimulate follicles to produce progesterone. At 96 h after mating, the testosterone response to LH was again manifest. These results suggest that the responsiveness of rabbit follicles to LH is dependent on the endocrine status of the animal when the ovaries were removed.